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ĿоҺڱࣨPAEsʴлˮƽ11-ǻ̴ø11-HSD2øԵĹ̽PAEsӰ̥ڷĿܻơ2012õȡڷٻIUGR33ƥ33øЧҺɫ-׷ⶨҺ4PAEsлڱᵥMBPڱᣨ2-һMEHPڱᣨ2-һ-5ǻ(MEHHP)ڱᣨ2-һ-5MEOHP3ƤʴƤͪлƤʴTHFallo-Ƥʴ(allo-THF)ƤͪTHEĺҺPAEsлˮƽ11-HSD2øԵĹ66ҺMBPMEHPMEHHPMEOHPлļʷֱΪ98%6589%5991%6091%60ĸҺMBPMEHHPMEOHPDEHPϼƣSumDEHPλֱΪ31.2024.6111.7248.67 ng/ml,ڶ飨ֱΪ17.3212.035.6828.64 ng/mlͳѧ壨Pֵ<0.05ĸ11-HSD2Ļԣ(THF+allo-THF)/THE=0.790.09 ng/mlݵڶ(THF+allo-THF)/THE=0.580.04 ng/mlݣӤĸҺMBP(¡=1.12)MEHHP(¡=1.14)MEOHP(¡=1.10)SumDEHP(¡=1.08)ĺ11-HSD2ĻԳʸأͳѧ壨Pֵ<0.05 PAEsͨ11-HSD2ĻӰ̥ڷ

ؼʣ̥ٻƤʴڱڷٻ11ªǻ̴ø

Abstract:

ObjectiveTo study the association between phthalate esters (PAEs)  metabolites in maternal urine and 11beta-hydroxysteroid dehydrogenase type 211-HSD2 enzyme activity,  explore the possible mechanism of PAEs effect on fetal development. MethodsAll of 33 cases of intrauterine growth retardation (IUGR) newborn were selected by random sampling in 2012. And 33 cases of normal control newborn were enrolled, use high performance liquid chromatography-tandem mass spectrometry method was used to detect 4 kinds of phthalate esters (PAEs)  metabolites  in maternal urinemono-n-butyl phthalate ester (MBP), mono (2-ethylhexyl) phthalate (MEHP), mono (2-ethyl-5-hydroxyhexyl) phthalate(MEHHP), mono (2-ethyl-5-oxohexyl) phthalate (MEOHP) and three kinds of cortisol corticosterone metabolites,tetrahydrocortisol (THF), allo-tetrahydrocortisol (allo-THF), tetrahydrocortisone (THE), and analyze the association between phthalate esters (PAEs)  metabolites in maternal urine and 11-HSD2 enzyme activity. ResultsMBPMEHPMEHHPMEOHP metabolites can be detected in 98%65 cases,89%59 cases,91%60 cases,91%60 casesof all 66 maternal urine samples, respectively. The median concentrations of test material in case group were 31.20 ng/ml for MBP24.61 ng/ml for MEHHP11.72 ng/ml for MEOHP and 48.67 ng/ml for SumDEHP which were significantly higher than those of the control groupwere 17.32,12.03,5.68 and 28.64 ng/ml; 11-HSD2 activity in case group(THF+allo-THF)/THE=(0.790.09) ng/mlwas significantly lower than that of the control group((THF+allo-THF)/THE=(0.580.04) ng/ml);  PAEs metabolites MBP(¡=1.12),MEHHP(¡=1.14),MEOHP(¡=1.10),SumDEHP(¡=1.08) in baby boy mothers urine was reversely correlated to 11-HSD2 activity. ConclusionsPAEs could affect fetal development by inhibit 11-HSD2 activity.

Key words: Fetal growth retardationTetrahydrocortisolPhthalate esters;Intrauterine retarded;11ªhydroxy steroid dehydrogenase

ڣ2014/9

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