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纳米二氧化钛短期经口染毒对幼年大鼠骨髓细胞的遗传损伤作用
作者:王云 陈章健 巴特 濮吉 顾永恩 郭健 贾光

摘要:

目的  探讨纳米二氧化钛灌胃染毒对幼年大鼠骨髓细胞的遗传损伤作用。方法  将28只清洁级雄性SD幼年大鼠(4周龄),按体重以随机数字表法分为4组(每组7只),每天1次灌胃,分别给予0、10、50、200 mg/kg纳米二氧化钛[(75±15) nm,锐钛矿],染毒30 d后取骨髓细胞涂片,进行微核试验和γ–H2AX免疫荧光检测。结果  50 mg/kg纳米二氧化钛染毒组大鼠的γ–H2AX焦点阳性细胞率[(37.4±10.0)%],明显高于对照组[(19.8±3.1)%],差异有统计学意义(t=–17.59,P<0.01)。各染毒组大鼠与对照组相比,骨髓嗜多染红细胞与正染红细胞的比值(PCE/NCE)和嗜多染红细胞微核率无明显变化。结论  短期经口摄入纳米二氧化钛,可导致幼年大鼠骨髓细胞DNA双链断裂增加,但对骨髓细胞微核无明显影响。

关键词:纳米粒;钛;大鼠;DNA损伤;安全

Abstract:

Objective  To explore the genotoxic effects of oral-exposed TiO2 nanoparticles on bone marrow cells in young rats.Methods  Twenty-eight SD male young rats (4 weeks old) were randomly divided into 4 groups, which were exposed to TiO2 nanoparticles ((75±15) nm, anatase) through intragastric administration at 0, 10, 50 and 200 mg/kg body weight (bw) every day for 30 days. The bone marrow cells were collected for micronuclei and γ-H2AX immunofluorescence analysis.Results  The percentage of γ-H2AX foci-positive cells (37.4±10.0)% in the 50 mg/kg bw dose group were significantly higher than that in the control group (19.8±3.1)% (t value was -17.59, P<0.01). No significantly difference was found in polychromatic erythrocyte/normochromatic erythrocyte (PCE/NCE) ratio and PCE micronucleus rate between three experimental groups and control group.Conclusion  TiO2 nanoparticles can increase the frequency of DNA double-strand breaks in bone marrow cells, but has no effect on micronucleus of bone marrow cells in young rats.

Key words: Nanoparticles;Titanium;Rats;DNA damage;Safety

发表日期:2014/9

引用本文:

图/表:

  • 10.3760/cma.j.issn.0253-9624.2014.09.014.T001:表1 纳米二氧化钛对幼年大鼠PCE/NCE比值的影响(±s,n=7)

    10.3760/cma.j.issn.0253-9624.2014.09.014.T001:表1 纳米二氧化钛对幼年大鼠PCE/NCE比值的影响(±s,n=7)

  • 10.3760/cma.j.issn.0253-9624.2014.09.014.T002:表2 纳米二氧化钛对幼年大鼠骨髓嗜多染红细胞微核率的影响(±s,n=7)

    10.3760/cma.j.issn.0253-9624.2014.09.014.T002:表2 纳米二氧化钛对幼年大鼠骨髓嗜多染红细胞微核率的影响(±s,n=7)

  • 10.3760/cma.j.issn.0253-9624.2014.09.014.T003:表3 纳米二氧化钛诱导幼年大鼠骨髓细胞γ–H2AX焦点的形成(±s,n=3)

    10.3760/cma.j.issn.0253-9624.2014.09.014.T003:表3 纳米二氧化钛诱导幼年大鼠骨髓细胞γ–H2AX焦点的形成(±s,n=3)

参考文献:

[1]李英杰, 白明. 二氧化钛的特性及在食品中的应用[J]. 食品安全导刊, 2010(8):58–59.
[2]WeirA, WesterhoffP, FabriciusL, et al. Titanium dioxide nanoparticles in food and personal care products[J]. Environ Sci Technol, 2012, 46(4):2242–2250.
[3]ChenXX, ChengB, YangYX, et al. Characterization and preliminary toxicity assay of nano–titanium dioxide additive in sugar–coated chewing gum[J]. Small, 2013, 9(9/10):1765–1774.
[4]RashidiL, Khosravi–DaraniK. The applications of nanotechnology in food industry[J]. Crit Rev Food Sci Nutr, 2011, 51(8):723–730.
[5]CardJW, JonaitisTS, TafazoliS, et al. An appraisal of the published literature on the safety and toxicity of food–related nanomaterials[J]. Crit Rev Toxicol, 2011, 41(1):20–49.
[6]王云, 巴特, 陈章健, 等. 纳米二氧化钛对胃溃疡大鼠血象的影响[J]. 中华预防医学杂志, 2012, 46(8):740–744.
[7]WangY, ChenZ, BaT, et al. Susceptibility of young and adult rats to the oral toxicity of titanium dioxide nanoparticles[J]. Small, 2013, 9(9/10):1742–1752.
[8]LomerMC, HutchinsonC, VolkertS, et al. Dietary sources of inorganic microparticles and their intake in healthy subjects and patients with Crohn′s disease[J]. Br J Nutr, 2004, 92(6):947–955.
[9]TrouillerB, RelieneR, WestbrookA, et al. Titanium dioxide nanoparticles induce DNA damage and genetic instability in vivo in mice[J]. Cancer Res, 2009, 69(22):8784–8789.
[10]SychevaLP, ZhurkovVS, IurchenkoVV, et al. Investigation of genotoxic and cytotoxic effects of micro– and nanosized titanium dioxide in six organs of mice in vivo[J]. Mutat Res, 2011, 726(1):8–14.

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