首页 >>2016年03期
浙江省冬春季涉禽场所禽流感病毒污染特征及影响因素研究
作者:王笑笑 程伟 余昭 茅海燕 陈恩富

摘要:

目的  分析浙江省涉禽场所外环境A型禽流感病毒污染特征及活禽市场被H7亚型污染的影响因素。方法  2014年10月至2015年3月,于浙江省11个市的涉禽场所进行标本采集,开展禽流感病毒污染状况监测。采集的标本类型包括涉禽场所的粪便标本、笼具表面擦拭标本、禽类饮水、清洗禽类的污水、宰杀或摆放禽肉案板表面擦拭标本及其他标本。本研究共采集标本6 457份,其中城乡活禽市场采集4 487份,家禽规模养殖场(户)820份、家禽散养户715份、家禽屠宰加工厂118份、野生禽鸟栖息地118份及其他场所86份。采用χ2检验比较不同类型或特征活禽市场、不同类型标本各亚型的核酸阳性率差异。采用多因素非条件二分类logistic回归模型分析活禽市场被H7亚型病毒污染的相关因素。结果  6 457份标本中,A型病毒核酸阳性率为32.54%(2 101份),其中H5亚型核酸阳性率为3.67%(237份),H7亚型为12.02%(776份),H9亚型为11.77%(760份)。237个活禽市场中,H7亚型核酸阳性的占33.8%(80个)。家禽屠宰加工厂A型流感病毒核酸阳性率较高,为43.72%(101/231)(χ2=737.80,P<0.001),城乡活禽市场H5/H7/H9亚型核酸阳性率较高,为27.55%(1 236/4 487)(χ2=436.37,P<0.001)。与售卖1种活禽的活禽市场相比,售卖≥2种活禽的市场被H7亚型感染的OR(95%CI)值为4.58(1.63~12.87)。结论  活禽市场和家禽屠宰加工厂等场所的A型禽流感病毒污染严重,售卖活禽种类较多可能是活禽市场被H7亚型污染的相关因素。

关键词:禽流感;病毒;影响因素

Abstract:

Objective  To evaluate the prevalence of avian influenza virus in various environment and the influence factors for subtype H7 prevalence in live poultry markets.Methods  We collected environmental samples from various environments across 11 cities of Zhejiang province between October 2014 and March 2015. Cage surface swabs, chopping board surface swabs, feces, water for cleaning, drinking water and swabs of other surfaces were collected. A total of 6 457 samples were collected, including 4 487 samples from poultry markets, 820 samples from poultry farms, 715 samples from backyard poultry pens, 118 samples from poultry processing factories, 118 samples from wild bird habitats and 86 samples from other sites. The chi-squared test was used to compare virus prevalence among sample types, sites types, and poultry markets types. Binary logistic regression was used to analyze factors on H7 subtype prevalence in poultry markets.Results  Of 6 457 samples, 32.54% (2 101) samples were positive for avian influenza, with 3.67% (237) positive for H5 subtype, 12.02%(776) positive for H7 subtype, 11.77%(760) positive for H9 subtype. Of 237 live poultry markets, 33.8% (80) were positive for H7 subtype. The prevalence of influenza A in poultry processing factories was the highest at 43.72% (101/231) (χ2=737.80, P<0.001). Poultry markets were contaminated most seriously by subtype H5/H7/H9 with the prevalence of 27.55% (1 236/4 487) (χ2=436.37, P<0.001). Compared with markets with 1 type of poultry, OR was 4.58 (95%CI: 1.63-12.87) for markets with ≥2 types of poultry.Conclusion  Live poultry markets and poultry processing factories were contaminated most seriously by avian influenza. The types of poultry might be the factor which influenced the subtype H7 prevalence in poultry markets.

Key words: Influenza in birds;Viruses;Risk factors

发表日期:2016/3

引用本文:

图/表:

  • 10.3760/cma.j.issn.0253-9624.2016.03.012.T001:表1 不同类型涉禽场所禽流感病毒各亚型检出情况比较[n(%)]

    10.3760/cma.j.issn.0253-9624.2016.03.012.T001:表1 不同类型涉禽场所禽流感病毒各亚型检出情况比较[n(%)]

  • 10.3760/cma.j.issn.0253-9624.2016.03.012.T002:表2 不同类型标本禽流感病毒各亚型核酸检出情况比较[n(%)]

    10.3760/cma.j.issn.0253-9624.2016.03.012.T002:表2 不同类型标本禽流感病毒各亚型核酸检出情况比较[n(%)]

  • 10.3760/cma.j.issn.0253-9624.2016.03.012.T003:表3 不同特征活禽市场A型禽流感病毒H7亚型核酸阳性率比较(n=237)

    10.3760/cma.j.issn.0253-9624.2016.03.012.T003:表3 不同特征活禽市场A型禽流感病毒H7亚型核酸阳性率比较(n=237)

  • 10.3760/cma.j.issn.0253-9624.2016.03.012.T004:表4 影响活禽市场被H7亚型禽流感病毒污染的多因素logistic回归分析

    10.3760/cma.j.issn.0253-9624.2016.03.012.T004:表4 影响活禽市场被H7亚型禽流感病毒污染的多因素logistic回归分析

参考文献:

[1]LiQ, ZhouL, ZhouM, et al. Epidemiology of human infections with avian influenza A(H7N9) virus in China[J]. N Engl J Med, 2014, 370(6):520-532. DOI: 10.1056/NEJMoa1304617.
[2]褚维,宋黎黎,劳宝法,等.H7N9禽流感监测病例的病原谱分析[J].中华预防医学杂志,2013,47(12):1153-1154.DOI:10.3760/cma.j.issn.0253-9624.2013.12.018.
[3]ChenE, WangF, LvH, et al. The first avian influenza A (H7N9) viral infection in humans in Zhejiang Province, China: a death report[J]. Front Med, 2013, 7(3):333-344. DOI: 10.1007/s11684-013-0275-1.
[4]丁华,谢立,考庆君,等.杭州市首例人感染H7N9禽流感病例调查[J].中华预防医学杂志,2013,12(47):1155-1156.DOI: 10.3760/cma.j.issn.0253-9624.2013.12.019.
[5]YuH, WuJT, CowlingBJ, et al. Effect of closure of live poultry markets on poultry-to-person transmission of avian influenza A H7N9 virus: an ecological study[J]. Lancet, 2014, 383(9916):541-548. DOI: 10.1016/S0140-6736(13)61904-2.
[6]Pantin-JackwoodMJ, MillerPJ, SpackmanE, et al. Role of poultry in the spread of novel H7N9 influenza virus in China[J]. J Virol, 2014, 88(10):5381-5390. DOI: 10.1128/JVI.03689-13.
[7]KhalenkovA, LaverWG, WebsterRG. Detection and isolation of H5N1 influenza virus from large volumes of natural water[J]. J Virol Methods, 2008, 149(1):180-183. DOI: 10.1016/j.jviromet.2008.01.001.
[8]SamaanG, GultomA, IndrianiR, et al. Critical control points for avian influenza A H5N1 in live bird markets in low resource settings[J]. Prev Vet Med, 2011, 100(1):71-78. DOI: 10.1016/j.prevetmed.2011.03.003.
[9]IndrianiR, SamaanG, GultomA, et al. Environmental sampling for avian influenza virus A (H5N1) in live-bird markets, Indonesia[J]. Emerg Infect Dis, 2010, 16(12):1889-1895. DOI: 10.3201/eid1612.100402.
[10]HormSV, SornS, AllalL, et al. Influenza A(H5N1) virus surveillance at live poultry markets, Cambodia, 2011[J]. Emerg Infect Dis, 2013, 19(2):305-308. DOI: 10.3201/eid1902.121201.
[11]LeungYH, ZhangLj, ChowCK, et al. Poultry drinking water used for avian influenza surveillance[J]. Emerg Infect Dis, 2007, 13(9):1380-1382.DOI: 10.3201/eid1309.070517.
[12]田怀玉.气候变化是否会改变野鸟H5N1禽流感暴发风险[J].中华预防医学杂志,2014,12(48):1067.
[13]史景红,高立冬,张红,等.东洞庭湖地区野禽驯养繁殖基地的禽流感病毒调查[J],动物学杂志,2015,5(4):607-614.DOI: 10.13859/j.cjz.201504013.
[14]DuanL, ZhuH, WangJ, et al. Influenza virus surveillance in migratory ducks and sentinel ducks at Poyang Lake, China[J]. Influenza Other Respi Viruses, 2011, 5Suppl 1:65-68.
[15]刘慧,陈宗道,肖新才,等.广州地区活禽市场休市措施对控制禽流感病毒污染效果的评价[J].中华流行病学杂志,2014,35(7):832-837.DOI:10.3760/cma.j.issn.0254-6450.2014.07.017.
[16]YuanJ, TangX, YangZ, et al. Enhanced disinfection and regular closure of wet markets reduced the risk of avian influenza A virus transmission[J]. Clin Infect Dis, 2014, 58(7):1037-1038. DOI: 10.1093/cid/cit951.

用户评论 0条

用户名: 密码: 登陆 注册
 
  • 9
  • 3
  •  4 : 页次:0/0页 共0条记录 5条/每页
    关于我们 | 专家风采 | 会员注册 | 继续教育
    地 址:北京市西城区东河沿街69号正弘大厦511室 邮 编:100052
    电话:010-51322302
    版权所有 中华医学会及中华预防医学杂志编辑部
    京ICP备 07035254 号