首页 >>2016年03期
杭州市急性呼吸道感染婴幼儿中副流感病毒3型遗传进化及其感染特征研究
作者:钱昕 于新芬 赵敏 寇宇 李钧 周银燕

摘要:

目的  分析杭州市婴幼儿中副流感病毒3型(HPIV-3)的遗传进化及其感染与合并感染情况。方法  收集2009年12月至2013年3月杭州地区医院0~11岁婴幼儿急性呼吸道感染病例咽拭子或呼吸道吸出物样本,共856份(男性553例,女性303例)。采用逆转录荧光定量PCR(qRT-PCR)法检测HPIV-3,阳性样本用特异性引物扩增HN基因进行基因测序,将测序所得序列用Clustal X 2.0软件进行比对,然后使用MEGA 6.0软件,采用邻接法建立系统进化树。同时,用qRT-PCR法检测与其他呼吸道病原体的合并感染情况。采用χ2检验比较不同特征患儿单一感染与合并感染情况。结果  856例患儿中,HPIV-3阳性84例(9.6%)。男性患儿HPIV-3感染阳性率为10.0%(55/553),女性患儿阳性率为9.6%(29/303)(χ2=0.03,P=0.860)。84例HPIV-3阳性病例的合并感染率为49%(41例),其中与鼻病毒合并感染检出率较高,为41%(17例);1岁以下患儿的合并感染率为54%(36/67),高于单一感染率[46%(31/67)](χ2=4.78,P=0.029);单一感染患儿伴有肺炎症状占93%(37/40),高于合并感染组[76%(29/38)](χ2=3.92,P=0.048)。同源性分析结果显示,56例患儿HPIV-3的HN基因编码区核苷酸和氨基酸序列一致性均较高,一致性分别为96.9%~100.0%和98.6%~100%。基于HN基因的进化树分析表明,杭州株均归属于C3亚群。结论  杭州市婴幼儿的急性呼吸道感染与HPIV-3有关;HPIV-3 HN基因型变异较小,但进化具有一定地域特征;与其他呼吸道病毒的合并感染率较高。

关键词:副流感病毒3型,人;感染;遗传

Abstract:

Objective  To determine the level of genetic variation of human parainfluenza virus type 3 (HPIV-3), and to describe infection and co-infection characteristics of HPIV-3 in children.Methods  Single respiratory samples from 856 pediatric patients with acute respiratory tract infection (ARI) in Hangzhou were collected from December 2009 to March 2013. All samples were screened for HPIV-3 by real-time RT-PCR and followed by HN sequencing and phylogenetic analysis. In all RSV positive specimens, we screened for the other pathogens, and co-infection characteristics were evaluated.Results  A total of 9.6% of 856 samples were positive for HPIV-3, the nucleotide among the strains ranged from 96.9% to 100%. All Hangzhou strains were placed in C3 subgroup based on HN gene analysis. 49% (n=41) of all HPIV-3-positive children with ARI were found to be co-infected with at least one of the other pathogen. The highest co-infection rate of HPIV-3 was with HRV (n=17). Children in the younger groups (≤12 months old) were significantly more prone to be co-infected with other pathogen (χ2=4.78, P=0.029). Pneumonia infection rate was significantly higher in the mono-infection group than the co-infection group (χ2=3.92, P=0.048).Conclusion  HPIV-3 was an important pathogen in children with ARI in Hangzhou. HN gene variation rate was low, but showed a more local pattern. The co-infections with other respiratory viruses were popular. Except for pneumonia, no significant differences in other clinical presentation between the HPIV-3 mono-infection and co-infection groups were observed.

Key words: Parainfluenza virus 3, human;Infection;Heredity

发表日期:2016/3

引用本文:

图/表:

  • 10.3760/cma.j.issn.0253-9624.2016.03.013.T001:表1 不同类型PCR使用的引物序列及产物长度

    10.3760/cma.j.issn.0253-9624.2016.03.013.T001:表1 不同类型PCR使用的引物序列及产物长度

  • 10.3760/cma.j.issn.0253-9624.2016.03.013.T002:表2 杭州市副流感病毒3型阳性患儿与其他病毒合并感染情况

    10.3760/cma.j.issn.0253-9624.2016.03.013.T002:表2 杭州市副流感病毒3型阳性患儿与其他病毒合并感染情况

  • 10.3760/cma.j.issn.0253-9624.2016.03.013.T003:表3 杭州市单一与合并感染副流感病毒3型患儿的临床特征比较

    10.3760/cma.j.issn.0253-9624.2016.03.013.T003:表3 杭州市单一与合并感染副流感病毒3型患儿的临床特征比较

  • 10.3760/cma.j.issn.0253-9624.2016.03.013.F001:图1 杭州市22株副流感病毒3型分离株基于21株血凝素-神经氨酸酶基因参考株的系统进化树

    10.3760/cma.j.issn.0253-9624.2016.03.013.F001:图1 杭州市22株副流感病毒3型分离株基于21株血凝素-神经氨酸酶基因参考株的系统进化树

  • 10.3760/cma.j.issn.0253-9624.2016.03.013.T004:表4 杭州市副流感病毒3型分离株与原型株血凝素-神经氨酸酶基因氨基酸变异位点比较

    10.3760/cma.j.issn.0253-9624.2016.03.013.T004:表4 杭州市副流感病毒3型分离株与原型株血凝素-神经氨酸酶基因氨基酸变异位点比较

参考文献:

[1]MosconaA. Entry of parainfluenza virus into cells as a target for interrupting childhood respiratory disease[J]. J Clin Invest, 2005, 115(7):1688-1698.
[2]LaurichesseH, DedmanD, WatsonJM, et al. Epidemiological features of parainfluenza virus infections: laboratory surveillance in England and Wales, 1975-1997[J]. Eur J Epidemiol, 1999, 15(5):475-484.
[3]王宇清,季伟.呼吸道常见病毒和新型病毒与儿童呼吸道感染的关系[J].中华预防医学杂志,2011,45(3):266-269.DOI: 10.3760/cma.j.issn.0253-9624.2011.03.016.
[4]van Wyke CoelinghKL, WinterCC, JorgensenED, et al. Antigenic and structural properties of the hemagglutinin-neuraminidase glycoprotein of human parainfluenza virus type 3: sequence analysis of variants selected with monoclonal antibodies which inhibit infectivity, hemagglutination, and neuraminidase activities[J]. J Virol, 1987, 61(5):1473-1477.
[5]RaymondF, CarbonneauJ, BoucherN, et al. Comparison of automated microarray detection with real-time PCR assays for detection of respiratory viruses in specimens obtained from children[J]. J Clin Microbiol, 2009, 47(3):743-750. DOI: 10.1128/JCM.01297-08.
[6]YuXF, PanJC, YeR, et al. Preparation of armored RNA as a control for multiplex real-time reverse transcription-PCR detection of influenza virus and severe acute respiratory syndrome coronavirus[J]. J Clin Microbiol, 2008, 46(3):837-841.
[7]DamenM, MinnaarR, GlasiusP, et al. Real-time PCR with an internal control for detection of all known human adenovirus serotypes[J]. J Clin Microbiol, 2008, 46(12):3997-4003.
[8]赵林清,钱渊,王芳,等.北京地区急性呼吸道感染婴幼儿中人副流感病毒感染状况的研究[J].中华儿科杂志,2007,45(2):91-95.DOI:10.3760/j.issn:0578-1310.2007.02.003.
[9]季伟,陈正荣,郭红波,等.苏州儿童医院住院儿童呼吸道病毒的流行特点及与气候因素的相关性研究[J].中华预防医学杂志, 2011, 45(3):205-210.DOI:10.3760/cma.j.issn.0253-9624.2011.03.004.
[10]胡燕,王宏萍,张璐,等.上海市某医院急性呼吸道感染儿童中人3型副流感病毒的检测及进化树分析[J].中华传染病杂志, 2014, 32(8):455-459. DOI: 10.3760/cma.j.issn.1000-6680.2014.08.002.
[11]MaoN, JiY, XieZ, et al. Human parainfluenza virus-associated respiratory tract infection among children and genetic analysis of HPIV-3 strains in Beijing, China[J]. PLos One, 2012, 7(8):e43893. DOI: 10.1371/journal.pone.0043893.
[12]HaradaY, KinoshitaF, YoshidaLM, et al. Does respiratory virus coinfection increases the clinical severity of acute respiratory infection among children infected with respiratory syncytial virus?[J]. Pediatr Infect Dis J, 2013, 32(5):441-445. DOI: 10.1097/INF.0b013e31828ba08c.
[13]FranzA, AdamsO, WillemsR, et al. Correlation of viral load of respiratory pathogens and co-infections with disease severity in children hospitalized for lower respiratory tract infection[J]. J Clin Virol, 2010, 48(4):239-245. DOI: 10.1016/j.jcv.2010.05.007.
[14]AsnerSA, RoseW, PetrichA, et al. Is virus coinfection a predictor of severity in children with viral respiratory infections?[J]. Clin Microbiol Infect, 2015, 21(3):261-264. DOI:10.1016/j.cmi.2014.08.024.
[15]YangHT, JiangQ, ZhouX, et al. Identification of a natural human serotype 3 parainfluenza virus[J]. Virol J, 2011, 8:58. DOI: 10.1186/1743-422X-8-58.
[16]LawrenceMC, BorgNA, StreltsovVA, et al. Structure of the haemagglutinin-neuraminidase from human parainfluenza virus type III[J]. J Mol Biol, 2004, 335(5):1343-1357. DOI: 10.1016/j.jmb.2003.11.032.
[17]MizutaK, TsukagoshiH, IkedaT, et al. Molecular evolution of the haemagglutinin-neuraminidase gene in human parainfluenza virustype 3 isolates from children with acute respiratory illness in Yamagata prefecture, Japan[J]. J Med Microbiol, 2014, 63(Pt 4):570-577. DOI: 10.1099/jmm.0.068189-0.

用户评论 0条

用户名: 密码: 登陆 注册
 
  • 9
  • 3
  •  4 : 页次:0/0页 共0条记录 5条/每页
    关于我们 | 专家风采 | 会员注册 | 继续教育
    地 址:北京市西城区东河沿街69号正弘大厦511室 邮 编:100052
    电话:010-51322302
    版权所有 中华医学会及中华预防医学杂志编辑部
    京ICP备 07035254 号