首页 >>2020年02期
中国破伤风抗体检测技术研究进展
作者:王传林 李明 吕新军

摘要:

我国非新生儿破伤风防控形势严峻,强化含破伤风类毒素疫苗(TTCV)的主动免疫是非新生儿破伤风防控的关键。通过破伤风抗体(TAB)检测可以明确个体的TTCV免疫状态,从而正确实施TTCV主动免疫。国外对TAB检测技术的研究呈现停滞状态,而国内有现实的TAB检测需求,破伤风抗体检测技术仍然处于动态发展过程中。本文收集国内也相对有限的TAB检测技术资料,对国内应用和发展的小鼠毒素中和试验(MTNT)、间接血凝法(IHA)、凝胶双向扩散试验或Rubin法、酶联免疫吸附试验(ELISA)、胶体金(CG)法等TAB检测技术进行总结,以期为国内TAB检测提供较全面的依据。由于缺乏国内TAB检测和国际机构、参考试剂的对比研究,我国的TAB检测技术尚未实现国际化认可。我国特殊的破伤风防控形势使得TAB检测技术研究在国内有一定的现实意义,ELISA、CG法等快速检测试剂具备一定的应用价值。

关键词:破伤风类毒素;疫苗;抗体;酶联免疫吸附试验;胶体金

Abstract:

The situation of prevention of non-neonatal tetanus in China is severe. Strengthening the active immunization with tetanus toxoid vaccine (TTCV) is the key to prevent the non-neonatal tetanus. Through the detection of tetanus antibody (TAB), the immune status of individual can be determined, so as to implement the active immunization of TTCV correctly. The research on TAB detection technology is stagnant in aboard, but still in a development process in China since there is a realistic demand for TAB detection. This review collects relatively limited data of TAB detection technology in China, and summarizes the techniques such as mice toxin neutralization test (MTNT), indirect hemagglutination assay (IHA), double agar gel immune diffusion test (Rubin method), enzyme-linked immunosorbent assay (ELISA) and colloidal gold (CG), in order to provide a comprehensive basis for domestic TAB detection. The TAB detection technology in China has not yet achieved international recognition due to the lack of comparative study of domestic and international institutions and reference reagents. The special domestic situation of tetanus prevention makes the research of TAB detection technology have a certain practical significance, and rapid detection reagents such as ELISA and CG method have a certain application value in China.

Key words: Tetanus toxoid;Vaccine;Antibody;Enzyme-linked immunosorbent assay;Colloidal gold

发表日期:2020/2

引用本文:

图/表:

    暂无图表

参考文献:

[1]Tetanus vaccines:WHO position paper-February 2017[J]. Wkly Epidemiol Rec, 2017, 92(6): 53-76.
[2]张春焕,伍业健,贺晴.广州市2016年人群破伤风抗体水平监测结果分析[J].热带医学杂志,2018,18(9):1242-1245. DOI: 10.3969/j.issn.1672-3619.2018.09.030.
[3]陈羽婷,马慧敏,熊号峰,等. 48例成人破伤风患者的临床特点及救治分析[J].北京医学,2018,40(7):683-686. DOI: 10.15932/j.0253-9713.2018.07.022.
[4]中国创伤救治联盟,北京大学创伤医学中心.中国破伤风免疫预防专家共识[J].中华外科杂志,2018,56(3):161-167. DOI: 10.3760/cma.j.issn.0529-5815.2018.03.001.
[5]王晗,于蕊,于长明,等.人破伤风基因工程中和抗体的研究进展[J].中华微生物和免疫学杂志, 2013, 33(5):379-384. DOI:10.3760/cma.j.issn.0254-5101.2013.05.016.
[6]国家药典委员会.中华人民共和国药典(三部)[M]. 2010版.北京:中国医药科技出版社, 2010.
[7]PerryAL, HayesAJ, CoxHA, et al. Comparison of five commercial anti-tetanus toxoid immunoglobulin G enzyme-linked immunosorbent assays[J]. Clin Vaccine Immunol, 2009, 16(12):1837-1839. DOI:10.1128/CVI.00294-09.
[8]GentiliG, PiniC, CollottiC. The use of an immunoenzymatic assay for the estimation of tetanus antitoxin in human sera: a cpmparison with seroneutralization and indirect haemagglutination[J]. J Biol Stand, 1985, 13(1):53-59. DOI:10.1016/S0092-1157(85)80033-0.
[9]张国强,冯素英,王晋,等.酶联免疫破伤风抗体定量试剂的研制及应用[J].微生物学免疫学进展,2006,34(2):23-27. DOI: 10.3969/j.issn.1005-5673.2006.02.006.
[10]RubinME, SayedHI, BowmanJM. Determination of tetanus anti-toxin levels in hyperimmunized volunteers[J]. Vox Sang, 1980, 38 (1): 6. DOI:10.1111/j.1423-0410.1980.tb02323.x.
[11]丁玉江,江昭伟,庞日强,等.一种检测人破伤风免疫血浆特异性抗体的快速准确方法[J].中国输血杂志,2005,18(6): 475-477. DOI: 10.3969/j.issn.1004-549X.2005.06.008.
[12]李荣贞,赵军,李庆英,等.酶联免疫法与中和实验法检测破伤风抗体效价[J].药学研究,2015,(7):381-383.
[13]丰达星,张珍英,徐瑾,等.破伤风抗体(IgG)定量检测试剂盒(酶联免疫法)使用评价[J].中外健康文摘,2011,8(46):18-20. DOI: 10.3969/j.issn.1672-5085.2011.46.011.
[14]张燕,叶小书,陈寒柏,等.破伤风类毒素抗体酶联双抗原夹心法定量检测试剂的研制及评价[J].微生物学免疫学进展, 2007, 35 (2):15-18. DOI: 10.3969/j.issn.1005-5673.2007.02.005.
[15]BorrowR, BalmerP, RoperMH, et al. The immunological basis for immunization series module 3: tetanus update 2006 [EB/OL]. [2019-01-01]. http://bases.bireme.br/cgi-bin/wxislind.exe/iah/online/?IsisScrip]iah/iah.xis&src=google&base=WHOLIS&lang=p&nextActio]lnk&exprSearch=i9241595551&indexSearch=ID.
[16]马霄,田霖,赵建荣,等.破伤风抗体胶体金检测试剂盒的研制[J].中国生物制品学杂志,2003,16(6):353-355. DOI: 10.3969/j.issn.1004-5503.2003.06.012.
[17]佟立波,王友豪,车吉泊,等.某部2016年新兵破伤风抗体水平流行病学调查[J].中国疫苗和免疫, 2018, 24 (1): 53-56.
[18]宁桂军. 2012年美国免疫实施咨询委员会关于≥65岁成人使用破伤风类毒素降低抗原含量的白喉类毒素和无细胞百日咳联合疫苗的最新建议[J].中国疫苗和免疫, 2013, 19(2): 190-191.

用户评论 0条

用户名: 密码: 登陆 注册
 
  • 9
  • 3
  •  4 : 页次:0/0页 共0条记录 5条/每页
    关于我们 | 专家风采 | 会员注册 | 继续教育
    地 址:北京市西城区东河沿街69号正弘大厦511室 邮 编:100052
    电话:010-51322302
    版权所有 中华医学会及中华预防医学杂志编辑部
    京ICP备 07035254 号