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2013—2017年广州市住院儿童呼吸道合胞病毒流行特征及分子生物学分析
作者:邹丽容 李振翠 钟志锋 梁丽君 宋颖超 武婕

摘要:

目的  分析广州人呼吸道合胞病毒(HRSV)的流行特征及遗传变异特征。方法  2013—2017年在广州两家哨点医院(广东省妇幼保健院和中山大学孙逸仙纪念医院)采集0~6岁急性呼吸道感染住院儿童的鼻咽拭子作为标本。采用逆转录PCR和巢式PCR方法进行HRSV的检测和分型,通过MEGA 6.0、NetNGlyc 1.0等软件对HRSV序列进行基因亲缘性分析以及N-糖基化位点的预测。结果  共收集鼻咽试子标本1 225份,其中男性783份,女性442份;月龄MP25P75)为8(3,24)月。共检出HRSV儿童感染者209例(17.06%),其中HRSV-A为117例(55.98%),HRSV-B为92例(44.02%)。2岁以下儿童HRSV检出率为18.83%(196例),占总阳性标本的93.78%。209例检出HRSV儿童中,32例(15.31%)还感染了至少1种其他呼吸道病毒。进化树分析显示,62株HRSV-A属于ON1基因型,2株属于NA1基因型,53株HRSV-B全部属于BA基因型。G蛋白的第二高变区在氨基酸替换,终止密码子的使用以及糖基化位点方面均具有多态性。结论  广州2岁以下儿童是感染HRSV的高发人群,ON1为HRSV-A主导基因型,BA为HRSV-B的主导基因型。多样化的氨基酸替换,某些糖基化位点的缺失与插入,体现了G蛋白作为主要保护性抗原的多样性。

关键词:呼吸道合胞病毒,人;遗传变异;流行病学;基因型

Abstract:

Objective  To understand the genetic variation and epidemiological characteristics of human respiratory syncytial virus (HRSV) in Guangzhou.Methods  Nasopharyngeal swabs specimens were collected from 0-6 year old children hospitalized with acute respiratory infection, then HRSV was tested and genotyped by RT-PCR. Phylogenetic tree was bulit using MEGA 6.0 software. NetNGlyc 1.0 server was used to predict the potential N-linked glycosylation sites.Results  A total of 1 225 nasopharyngeal specimens were collected, including 783 males and 442 females. The median (P25, P75) age was 8 (3, 24) months. Among the 209 HRSV-positive cases (17.06%), 117 cases (55.98%) were HRSV-A and 92 cases (44.02%) were HRSV-B. The two distinct subgroups (HRSV-A and HRSV-B) alternately played dominant role to cause HRSV infection and exchange almost once every two years. The HRSV prevalence rate decreased with age. The HRSV-positive rate among children under 2 years old was 18.83% (196 cases), accounting for 93.78% of the total positive cases. There were 32 HRSV positive cases co-infected with at least one respiratory virus, with the co-infection rate of 15.31%. Phylogenetic tree analysis of the second hypervariable region (HVR2) of the G protein classified the HRSV-A specimens into ON1 (n=62) and NA1 (n=2) genotypes while all HRSV-B specimens belonged to BA genotype (n=53). The HVR2 of the G protein varied in using stop condon, amino acid substitutions, glycosylation sites.Conclusion  Children under 2 years old were the high risk population of HRSV infection in Guangzhou. ON1 genotype turned into a primary genetype of the HRSV-A subgroup while BA genotype dominated the HRSV-B subgroup. A greater diversification of amino acid substitutions, and some deletion and insertion of glycosylation sites embodied the polymorphism of G protein as main protective antigen.

Key words: Respiratory syncytial virus, human;Genetic variation;Epidemiology;Genetype

发表日期:2020/3

引用本文:

图/表:

  • 10.3760/cma.j.issn.0253-9624.2020.03.010.T001

    10.3760/cma.j.issn.0253-9624.2020.03.010.T001

  • 10.3760/cma.j.issn.0253-9624.2020.03.010.T002

    10.3760/cma.j.issn.0253-9624.2020.03.010.T002

  • 10.3760/cma.j.issn.0253-9624.2020.03.010.F001

    10.3760/cma.j.issn.0253-9624.2020.03.010.F001

  • 10.3760/cma.j.issn.0253-9624.2020.03.010.T003

    10.3760/cma.j.issn.0253-9624.2020.03.010.T003

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    10.3760/cma.j.issn.0253-9624.2020.03.010.T004

  • 10.3760/cma.j.issn.0253-9624.2020.03.010.F002:图2 2013—2017年分离自广州急性呼吸道感染住院儿童64株呼吸道合胞病毒A型G蛋白第二高变区核苷酸序列亲缘关系进化树

    10.3760/cma.j.issn.0253-9624.2020.03.010.F002:图2 2013—2017年分离自广州急性呼吸道感染住院儿童64株呼吸道合胞病毒A型G蛋白第二高变区核苷酸序列亲缘关系进化树

  • 10.3760/cma.j.issn.0253-9624.2020.03.010.F003:图3 2013—2017年分离自广州急性呼吸道感染住院儿童53株呼吸道合胞病毒B型G蛋白第二高变区核苷酸序列亲缘关系进化树

    10.3760/cma.j.issn.0253-9624.2020.03.010.F003:图3 2013—2017年分离自广州急性呼吸道感染住院儿童53株呼吸道合胞病毒B型G蛋白第二高变区核苷酸序列亲缘关系进化树

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