中华预防医学杂志    2014年 邻苯二甲酸酯暴露对产妇11β-羟基类固醇脱氢酶活性的影响    PDF     文章点击量:2765    
中华预防医学杂志2014年
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胡小娅 赵岩 陈北涛 梁园 李露茜 谢长明 张蕴晖 林振浪 谢爱兰 陈尚勤
邻苯二甲酸酯暴露对产妇11β-羟基类固醇脱氢酶活性的影响
中华预防医学杂志, 2014,(9)
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邻苯二甲酸酯暴露对产妇11β-羟基类固醇脱氢酶活性的影响
胡小娅 赵岩 陈北涛 梁园 李露茜 谢长明 张蕴晖 林振浪 谢爱兰 陈尚勤     
摘要: [摘要] 目的 研究产妇尿液中邻苯二甲酸酯类(PAEs)物质代谢物的水平与11β-羟基类固醇脱氢酶(11β-HSD2酶)活性的关联,初步探索PAEs影响胎儿宫内发育的可能机制。方法 2012年采用单纯随机抽样法抽取宫内发育迟缓(IUGR)新生儿33例,并匹配正常对照新生儿33名,采用高效液相色谱-串联质谱法测定产妇尿液中4种PAEs代谢物,即邻苯二甲酸单丁酯(MBP)、邻苯二甲酸(2-乙基己基)单酯(MEHP)、邻苯二甲酸(2-乙基-5羟基己基)单酯(MEHHP ) 、邻苯二甲酸(2-乙基-5氧己基)单酯(MEOHP)和3种皮质醇皮质酮代谢物,即四氢皮质醇(THF)、allo-四氢皮质醇(allo-THF) 、四氢皮质酮(THE)的含量,并分析产妇尿液中PAEs代谢物的水平与11β-HSD2酶活性的关联。结果 66名产妇尿液样本中MBP、MEHP、MEHHP、MEOHP代谢物的检出率分别为98%(65名)、89%(59名)、91%(60名)、91%(60名);病例组母亲尿液样本中MBP、MEHHP、MEOHP和SumDEHP的中位数分别为31.20、24.61、11.72和48.67ng/ml,高于对照组(分别为17.32、12.03、5.68和28.64ng/ml),差异均具有统计学意义(P值均<0.05);病例组母亲体内11β-HSD2的活性[(THF+allo-THF)/THE=(0.79±0.09) ng/ml]低于对照组[(THF+allo-THF)/THE=(0.58±0.04) ng/ml];男婴母亲尿液中MBP(β=1.12)、MEHHP(β=1.14)、MEOHP(β=1.10)、SumDEHP(β=1.08)的含量与11β-HSD2的活性呈负相关,差异有统计学意义(P值均<0.05)。 结论 PAEs可能通过抑制11β-HSD2的活性影响胎儿宫内发育。
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